A Postdoctoral Research Assistant is required to work in Professor Manuela Zaccolo’s group. The post is ideally suited to a scientist with first-hand experience in proteomic and phosphoproteomic techniques applied to cardiac myocytes. Expertise in primary cardiac myocyte isolation and mice breeding and genotyping would be an advantage.
The post holder’s duties will include cardiomyocytes isolation and culture, the application of strategies for phosphopeptide enrichment, preparation of samples for mass spectrometry, and mass spectrometry data analysis using specialized software. Propagation and maintenance of virus and cell lines, PCR , cloning, immunoblotting and immunstaining; and the maintenance of breeding mouse colonies including genotyping and database management are also required.
The successful candidate must have a PhD/DPhil or equivalent in a relevant area of research and a strong background in biochemistry, protein purification and analysis, especially in regard to protein-protein interactions. Experience in mass spectrometry data analysis from heart tissue is also essential. Familiarity with the cAMP/PKA signaling pathway and previous experience in propagation and maintenance of virus and cell lines is desirable.
The post is based in the Dept of Physiology, Anatomy & Genetics, Sherrington Bldg, Parks Rd, Oxford OX1 3PT and funded by the BHF from 1 November 2012 until 31 July 2015. Please note that the successful candidate must be prepared to spend a period of up to 9 months at the Netherlands Proteomics Centre in Utrecht for sample running and date collection.
IMPORTANT : applications for this post must be made online. For a full job description and to apply, please visit:
https://www.recruit.ox.ac.uk/pls/hrisliverecruit/erq_jobspec_version_4.jobspec?p_id=103477
We cannot accept emailed applications for this post
Closing date midday on Wednesday 1 August 2012.
Interviews will be held in early September.
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